Journal: bioRxiv
Article Title: Type I Interferon Signaling Defines a Novel Disease Signature in Xeroderma Pigmentosum C Human Keratinocytes
doi: 10.1101/2025.07.17.665356
Figure Lengend Snippet: This heatmap presentation shows the differential expression profile of 413 proteins (arranged from the highly upregulated on the toplist to the downregulated on the bottom list according to the condition XPC KO IR vs WT IR used as a reference). Each row is a protein and each column corresponds to a specific condition starting from XPC KO UVB irradiated vs wild-type UVB irradiated (XPC KO IR vs WT IR), XPC KO UVB irradiated vs non-UVB irradiated (XPC KO IR vs XPC KO NIR), XPC KO non-UVB irradiated vs wild-type non-UVB irradiated (XPC KO NIR vs WT NIR), and wild-type UVB irradiated vs non-UVB irradiated (WT IR vs WT NIR). The 14 top list proteins selected according to the condition XPC KO IR vs WT IR compared to the other three conditions were Interferon-induced GTP-binding protein (MX1), Serpin Family B Member 3 (SERPINB3), S100 calcium-binding protein A12 (S100A12), Interferon Induced Protein With Tetratricopeptide Repeats 2 (IFIT-2), Interferon Induced Protein With Tetratricopeptide Repeats 3 (IFIT-3), Interferon Induced Protein With Tetratricopeptide Repeats 1 (IFIT-1), Interferon-induced GTP-binding protein (MX2), Suprabasin (SBSN), Interleukin 1 beta (IL-1β), S100 calcium-binding protein A7 (S100A7), Calmodulin Like 5 (CALML5), 2’-5’-Oligoadenylate Synthetase 1 (OAS1), Interferon Regulatory Factor 9 (IRF9), and Interferon-stimulated gene 15 (ISG15). The FunRich Version 3.1.4 was used to construct this heatmap. The Row Z score fluctuated from -1 to 1. *** p< 0.001, unpaired and paired t-test. The results presented are the mean of four biological replicates (N=4).
Article Snippet: The nitrocellulose membrane was blocked with 5% lyophilized milk or bovine serum album (BSA), followed by the addition of either primary XPC (Santa Cruz 1/500), IFIT-1 (abcam 1/500), IFIT-3 (Santa Cruz 1/500), IRF-9 (Santa Cruz 1/1000), ISG15 (Santa Cruz 1/500), MX1 (abcam 1/500), MX2 (abcam 1/500), PSTAT-1 Tyr701 (Cell Technologies 1/1000), PSTAT-2 Tyr690 (Cell Technologies 1/1000), PSTAT-3 Tyr705 (Cell Technologies 1/1000), antibody incubated overnight at 4°C.
Techniques: Quantitative Proteomics, Irradiation, Binding Assay, Construct
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